Nyren’s sequencing method earned the name “pyrosequencing” since it revolved around the production of pyrophosphate. At first, pyrosequencing could sequence only short DNA snippets, with a few nucleotides. In 1996, however, Nyren’s lab demonstrated sequencing of up to 15 bases by using a modified “A” nucleotide to reduce their signal-to-noise ratio.7 In 1998, they increased this to 34 bases by adding another enzyme, called apyrase, to the mix; apyrase degraded unincorporated nucleotides, removing the need for constant wash steps.
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温菲代表建议,要因地制宜加快出台传统制造业数智化转型专项规划,分行业、分阶段建立标准和评价体系,引导企业因业施策、精准发力。注重发挥龙头企业的带动作用,通过开放共享技术平台、共建工业互联网生态,帮助中小企业跨越“转型门槛”,让更多企业“敢转、愿转、会转”。要将数字化、智能化贯穿于研发设计、生产制造、经营管理、市场营销等全过程,推动全链条降本增效提质。
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第九十九条 因不可抗力或者其他不能归责于承运人和托运人的原因致使船舶不能在合同约定的目的港卸货的,除合同另有约定外,船长有权将货物在目的港邻近的安全港口或者地点卸载,视为已经履行合同。
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